Poplar Transformation.
Identifieur interne : 000771 ( Main/Exploration ); précédent : 000770; suivant : 000772Poplar Transformation.
Auteurs : Tobias Bruegmann [Allemagne] ; Olaf Polak [Allemagne] ; Khira Deecke [Allemagne] ; Julia Nietsch [Allemagne] ; Matthias Fladung [Allemagne]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2019.
Descripteurs français
- KwdFr :
- Agrobacterium (génétique), Biolistique (instrumentation), Biolistique (méthodes), Feuilles de plante (MeSH), Polyéthylène glycols (composition chimique), Populus (génétique), Techniques de culture de tissus (instrumentation), Techniques de culture de tissus (méthodes), Transformation génétique (MeSH), Vecteurs génétiques (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- composition chimique : Polyéthylène glycols.
- génétique : Agrobacterium, Populus, Vecteurs génétiques.
- méthodes : Biolistique, Techniques de culture de tissus.
- instrumentation : Biolistique, Feuilles de plante, Techniques de culture de tissus, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium (genetics), Biolistics (instrumentation), Biolistics (methods), Genetic Vectors (genetics), Plant Leaves (MeSH), Plants, Genetically Modified (MeSH), Polyethylene Glycols (chemistry), Populus (genetics), Tissue Culture Techniques (instrumentation), Tissue Culture Techniques (methods), Transformation, Genetic (MeSH).
- MESH :
- chemical , chemistry : Polyethylene Glycols.
- genetics : Agrobacterium, Genetic Vectors, Populus.
- instrumentation : Biolistics, Tissue Culture Techniques.
- methods : Biolistics, Tissue Culture Techniques.
- Plant Leaves, Plants, Genetically Modified, Transformation, Genetic.
Abstract
First publications of successful Agrobacterium-mediated transformation of tobacco were published more than 30 years ago. Protocols for Agrobacterium-based transformation as well as biolistic bombardment and PEG transformation of protoplasts are available for more than 150 plant species from various plant families. Also for many Populus species and hybrids, adapted transformation protocols have been published. The standard protocol for Agrobacterium-mediated transformation of different Populus genotypes is the leaf-disc method. Here, we first describe the transfer of genes into poplar by using the Agrobacterium-based leaf disc methods. In addition, alternative basic transformation methods, namely, biolistic bombardment and PEG transformation of protoplasts, are also described. Further, we present improved poplar transformation protocols by simplifying the transformation procedure and optimizing tissue preparation and plant regeneration.
DOI: 10.1007/978-1-4939-8778-8_12
PubMed: 30415336
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Protocols for Agrobacterium-based transformation as well as biolistic bombardment and PEG transformation of protoplasts are available for more than 150 plant species from various plant families. Also for many Populus species and hybrids, adapted transformation protocols have been published. The standard protocol for Agrobacterium-mediated transformation of different Populus genotypes is the leaf-disc method. Here, we first describe the transfer of genes into poplar by using the Agrobacterium-based leaf disc methods. In addition, alternative basic transformation methods, namely, biolistic bombardment and PEG transformation of protoplasts, are also described. Further, we present improved poplar transformation protocols by simplifying the transformation procedure and optimizing tissue preparation and plant regeneration.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30415336</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1864</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Poplar Transformation.</ArticleTitle><Pagination><MedlinePgn>165-177</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-8778-8_12</ELocationID><Abstract><AbstractText>First publications of successful Agrobacterium-mediated transformation of tobacco were published more than 30 years ago. Protocols for Agrobacterium-based transformation as well as biolistic bombardment and PEG transformation of protoplasts are available for more than 150 plant species from various plant families. Also for many Populus species and hybrids, adapted transformation protocols have been published. The standard protocol for Agrobacterium-mediated transformation of different Populus genotypes is the leaf-disc method. Here, we first describe the transfer of genes into poplar by using the Agrobacterium-based leaf disc methods. In addition, alternative basic transformation methods, namely, biolistic bombardment and PEG transformation of protoplasts, are also described. Further, we present improved poplar transformation protocols by simplifying the transformation procedure and optimizing tissue preparation and plant regeneration.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bruegmann</LastName><ForeName>Tobias</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Thuenen Institute of Forest Genetics, Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Polak</LastName><ForeName>Olaf</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Thuenen Institute of Forest Genetics, Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deecke</LastName><ForeName>Khira</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Thuenen Institute of Forest Genetics, Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nietsch</LastName><ForeName>Julia</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>CGS Crop Genetic Systems, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fladung</LastName><ForeName>Matthias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Thuenen Institute of Forest Genetics, Grosshansdorf, Germany. matthias.fladung@thuenen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>3WJQ0SDW1A</RegistryNumber><NameOfSubstance UI="D011092">Polyethylene Glycols</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019470" MajorTopicYN="N">Biolistics</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011092" MajorTopicYN="N">Polyethylene 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culture</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>6</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30415336</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-8778-8_12</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Bruegmann, Tobias" sort="Bruegmann, Tobias" uniqKey="Bruegmann T" first="Tobias" last="Bruegmann">Tobias Bruegmann</name></noRegion><name sortKey="Deecke, Khira" sort="Deecke, Khira" uniqKey="Deecke K" first="Khira" last="Deecke">Khira Deecke</name><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><name sortKey="Nietsch, Julia" sort="Nietsch, Julia" uniqKey="Nietsch J" first="Julia" last="Nietsch">Julia Nietsch</name><name sortKey="Polak, Olaf" sort="Polak, Olaf" uniqKey="Polak O" first="Olaf" last="Polak">Olaf Polak</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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